New 5D Biofilms Publication in Advances in Woundcare


Efficacy of Concentrated Surfactant-Based Wound Dressings in Wound Repair and Biofilm Reduction. 

Salisbury A-M, Mayer D, Chen R, Percival SL

Advances in Woundcare, April, 2018


Objective: This study was set up to evaluate the efficacy of a concentrated surfactant-based wound dressing (with and without silver sulfadiazine [SSD]) on wound repair, by investigating their ability to enhance human dermal fibroblast proliferation and viability. In addition, the wound dressings were evaluated for their ability to suppress biofilms in a three-dimensional (3D) in vitro wound biofilm model and modulate the inflammatory cytokine interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNFα).

Approach: Problematic biofilms are well known to affect fibroblast and keratinocyte viability. To assess wound repair and inflammatory cytokine modulation, a direct cytotoxicity assay and a 3D keratinocyte-fibroblast model were employed.

Results: At 1 and 7 days posttreatment, the non-antimicrobial dressing was noncytotoxic and the antimicrobial dressing was moderately cytotoxic to adult human dermal fibroblasts cells. Within the 3D wound model, the biofilm demonstrated a decelerating effect on wound closure and a decrease in viable cells. When the non-antimicrobial- and antimicrobial-based concentrated surfactant-based wound dressing was applied to the wound model, reduced biofilm was observed. The application of wound dressings to the biofilm-infected wound also resulted in a reduction of IL-6 and TNFα. The concentrated surfactant-based wound dressing without an antimicrobial was shown to enhance cellular viability and migration.

Innovation and Conclusion: We have demonstrated the ability of a surfactant-based wound dressing to minimize the deleterious effects of a wound biofilm, modulate the secretion of inflammatory cytokines, and enhance cellular proliferation in a biofilm-infected wound model. Furthermore, the non-antimicrobial-based concentrated surfactant dressings did not affect cellular viability and therefore represents a multifaceted approach to the treatment of wounds infected with biofilms.


New 5D Publication on Cell Salvage and Wounds 2018 13 SEP

New 5D Publication Cell salvage in acute and chronic wounds: a potential treatment str..

MIA Winner - 5D on Youtube 2018 18 AUG

Biofilm and microbiology firm wins MIA https://youtu.be/G81VSmz2h4w (https..

Read More News